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hepes buffered saline  (Thermo Fisher)


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    Structured Review

    Thermo Fisher hepes buffered saline
    Hepes Buffered Saline, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepes+buffered+saline/HEPES-buffered+saline%2C+pH+7%2E0/10__1021_slash_acsptsci__6c00082-85-7-17
    Average 95 stars, based on 1 article reviews
    hepes buffered saline - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cloning:

    Article Title: Epitope and HLA specificity of human TCRs against Plasmodium falciparum circumsporozoite protein
    Article Snippet: .. After cloning and sequence verification, Phoenix Ampho cells (American Type Culture Collection, catalog no. CRL-3213; RRID:CVCL_H716) were transfected with the TCR expression vectors using 2.5 M CaCl 2 and HEPES-buffered saline and cultured in DMEM GlutaMAX medium (Life Technologies) with 10% heat-inactivated FBS at 37°C and 5% CO 2 . ..

    Sequencing:

    Article Title: Epitope and HLA specificity of human TCRs against Plasmodium falciparum circumsporozoite protein
    Article Snippet: .. After cloning and sequence verification, Phoenix Ampho cells (American Type Culture Collection, catalog no. CRL-3213; RRID:CVCL_H716) were transfected with the TCR expression vectors using 2.5 M CaCl 2 and HEPES-buffered saline and cultured in DMEM GlutaMAX medium (Life Technologies) with 10% heat-inactivated FBS at 37°C and 5% CO 2 . ..

    Transfection:

    Article Title: Epitope and HLA specificity of human TCRs against Plasmodium falciparum circumsporozoite protein
    Article Snippet: .. After cloning and sequence verification, Phoenix Ampho cells (American Type Culture Collection, catalog no. CRL-3213; RRID:CVCL_H716) were transfected with the TCR expression vectors using 2.5 M CaCl 2 and HEPES-buffered saline and cultured in DMEM GlutaMAX medium (Life Technologies) with 10% heat-inactivated FBS at 37°C and 5% CO 2 . ..

    Expressing:

    Article Title: Epitope and HLA specificity of human TCRs against Plasmodium falciparum circumsporozoite protein
    Article Snippet: .. After cloning and sequence verification, Phoenix Ampho cells (American Type Culture Collection, catalog no. CRL-3213; RRID:CVCL_H716) were transfected with the TCR expression vectors using 2.5 M CaCl 2 and HEPES-buffered saline and cultured in DMEM GlutaMAX medium (Life Technologies) with 10% heat-inactivated FBS at 37°C and 5% CO 2 . ..

    Saline:

    Article Title: Epitope and HLA specificity of human TCRs against Plasmodium falciparum circumsporozoite protein
    Article Snippet: .. After cloning and sequence verification, Phoenix Ampho cells (American Type Culture Collection, catalog no. CRL-3213; RRID:CVCL_H716) were transfected with the TCR expression vectors using 2.5 M CaCl 2 and HEPES-buffered saline and cultured in DMEM GlutaMAX medium (Life Technologies) with 10% heat-inactivated FBS at 37°C and 5% CO 2 . ..

    Article Title: TUSC3 regulates ERMA-mediated Mg 2+ uptake for synaptic function and neurodevelopment.
    Article Snippet: .. To assess synaptic vesicle recycling, primary hippocampal neurons (DIV18) were first prewashed with pre-warmed HEPES-buffered saline (HBS; 135mM NaCl, 5mM KCl, 2mM CaCl2, 1mM MgCl2, 10mM glucose, and 10mM HEPES, pH 7.4) and then incubated for 5min in high K+ HBS (90mM KCl) containing 10μM FM1-43 dye (Invitrogen). ..

    Article Title: Mesenchymal stem cells derived from human bone marrow ameliorate monosodium urate crystal-induced inflammation.
    Article Snippet: Mesenchymal stem cells (MSCs) have anti-inflammatory properties and have been implicated in several inflammatory diseases.. Acute gout is initiated by IL-1β produced by monosodium urate crystal (MSU)-stimulated macrophages via activation of NLRP3 inflammasomes.. We sought to investigate whether MSCs derived from human bone marrow (hBM-MSCs) suppress MSU-induced inflammation. hBM-MSCs were stimulated with proinflammatory cytokine cocktails or co-cultured with MSU-treated macrophages.

    Article Title: A Simple and Cost-Effective Electrochemical Assay for Heart Failure Prognosis Using Soluble Suppression of Tumorigenicity 2-Specific Nanobodies
    Article Snippet: .. The final purified Nbs were buffer-exchanged into HEPES-buffered saline using 10 kDa molecular weight cutoff dialysis membranes (Thermo Fisher Scientific, USA). ..

    Article Title: Longitudinal assessment of functional antibodies to a novel influenza virus strain across age groups
    Article Snippet: Whole inactivated virus (WIV) was prepared by incubating the harvested and purified virus overnight with 0.1% (v/v) β-propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4 °C. .. The inactivated virus was subsequently dialyzed overnight at 4 °C against HEPES-buffered saline (Thermo Fisher Scientific, Bleiswijk, The Netherlands) to remove residual β-propiolactone. ..

    Article Title: An exploratory plasma-based functional assay for phenotypic characterization of fibrinolysis in dysfibrinogenemia
    Article Snippet: .. Fibrinogen precipitate was finally dissolved in 20 mM HEPES-buffered saline (pH 7.4) and dialyzed extensively against 20 mM HEPES-buffered saline (pH 7.4) using a Slide-A-Lyzer MINI dialysis device (20K MWCO; Thermo Fisher Scientific). ..

    Article Title: Molecular characterization of the archaic HLA-B∗73:01 allele reveals presentation of a unique peptidome and skewed engagement by KIR2DL2
    Article Snippet: .. The wells were then incubated with Protein L-HRP (Thermo Fisher Scientific) at a 1:5000 dilution in Hepes-buffered saline with Tween for 20 min. After another thorough washing step, the plates were developed using TMB substrate (Thermo Fisher Scientific). ..

    Article Title: Chromatin-opening element for stable long term gene expression
    Article Snippet: .. The total DNA was brought to 500 μL in water, to which 500 μL of 2×HEPES-buffered saline, pH7.0 (Alfa Aesar) was added and mixed. .. One-tenth of the total volume (100 μL) of 2.5 M calcium chloride (Sigma-Aldrich) was then added to the mixture, followed by a 20-minute incubation.

    Cell Culture:

    Article Title: Epitope and HLA specificity of human TCRs against Plasmodium falciparum circumsporozoite protein
    Article Snippet: .. After cloning and sequence verification, Phoenix Ampho cells (American Type Culture Collection, catalog no. CRL-3213; RRID:CVCL_H716) were transfected with the TCR expression vectors using 2.5 M CaCl 2 and HEPES-buffered saline and cultured in DMEM GlutaMAX medium (Life Technologies) with 10% heat-inactivated FBS at 37°C and 5% CO 2 . ..

    Incubation:

    Article Title: TUSC3 regulates ERMA-mediated Mg 2+ uptake for synaptic function and neurodevelopment.
    Article Snippet: .. To assess synaptic vesicle recycling, primary hippocampal neurons (DIV18) were first prewashed with pre-warmed HEPES-buffered saline (HBS; 135mM NaCl, 5mM KCl, 2mM CaCl2, 1mM MgCl2, 10mM glucose, and 10mM HEPES, pH 7.4) and then incubated for 5min in high K+ HBS (90mM KCl) containing 10μM FM1-43 dye (Invitrogen). ..

    Article Title: Molecular characterization of the archaic HLA-B∗73:01 allele reveals presentation of a unique peptidome and skewed engagement by KIR2DL2
    Article Snippet: .. The wells were then incubated with Protein L-HRP (Thermo Fisher Scientific) at a 1:5000 dilution in Hepes-buffered saline with Tween for 20 min. After another thorough washing step, the plates were developed using TMB substrate (Thermo Fisher Scientific). ..

    Purification:

    Article Title: A Simple and Cost-Effective Electrochemical Assay for Heart Failure Prognosis Using Soluble Suppression of Tumorigenicity 2-Specific Nanobodies
    Article Snippet: .. The final purified Nbs were buffer-exchanged into HEPES-buffered saline using 10 kDa molecular weight cutoff dialysis membranes (Thermo Fisher Scientific, USA). ..

    Molecular Weight:

    Article Title: A Simple and Cost-Effective Electrochemical Assay for Heart Failure Prognosis Using Soluble Suppression of Tumorigenicity 2-Specific Nanobodies
    Article Snippet: .. The final purified Nbs were buffer-exchanged into HEPES-buffered saline using 10 kDa molecular weight cutoff dialysis membranes (Thermo Fisher Scientific, USA). ..

    Virus:

    Article Title: Longitudinal assessment of functional antibodies to a novel influenza virus strain across age groups
    Article Snippet: Whole inactivated virus (WIV) was prepared by incubating the harvested and purified virus overnight with 0.1% (v/v) β-propiolactone (Acros Organics, Geel, Belgium) under continuous rotation at 4 °C. .. The inactivated virus was subsequently dialyzed overnight at 4 °C against HEPES-buffered saline (Thermo Fisher Scientific, Bleiswijk, The Netherlands) to remove residual β-propiolactone. ..



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